Journal: Frontiers in Pediatrics

Article Title: Detection of volatile organic compounds in headspace of Klebsiella pneumoniae and Klebsiella oxytoca colonies

doi: 10.3389/fped.2023.1151000

Figure Lengend Snippet: Box-and-whisker-Plot of the peaks P158 ( A ) and P51 ( B ). After Bonferroni post hoc analysis correction, Peaks P51 and P158 showed the highest sensitivity, specificity, positive and negative predictive value with 99.9% each in a significance level with p < 0.001 for K. pneumoniae (KP) when comparing KP and bare MacConkey agar plates (MC). Comparing K. oxytoca (KO) and MC P158 reaching the highest sensitivity, specificity, positive and negative predictive value of 99.9% each in a significance level with p < 0.001.

Article Snippet: Microbial cultures of KP and KO on MacConkey (MC) agar plates (Becton, Dickinson and Company, Franklin Lakes, New Jersey, USA) originated from rectal/anal swabs of preterm infants of the NICU of the Department of Pediatrics, Saarland University Medical Center, Homburg (Germany).

Techniques: Whisker Assay

Journal: Frontiers in Pediatrics

Article Title: Detection of volatile organic compounds in headspace of Klebsiella pneumoniae and Klebsiella oxytoca colonies

doi: 10.3389/fped.2023.1151000

Figure Lengend Snippet: Decision tree. A total of three peaks enabled the differentiation of K. pneumoniae (KP), K. oxytoca (KO) and bare MacConkey agar plates (MC) regarding their signal intensities. With a signal intensity ≤0.011 V, P158 allows to differentiate between bare MC, KP and KO, respectively. At a signal intensitiy >0.487 V, KP is identified via P108. Peak P22 enables further differentiation regarding a signal intensity ≤0.487 V: a signal intensity ≤0.010 V represents KO and a signal intensity >0.005 V represents KP.

Article Snippet: Microbial cultures of KP and KO on MacConkey (MC) agar plates (Becton, Dickinson and Company, Franklin Lakes, New Jersey, USA) originated from rectal/anal swabs of preterm infants of the NICU of the Department of Pediatrics, Saarland University Medical Center, Homburg (Germany).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Efficiency of N 2 Gas Flushing Compared to the Lactoperoxidase System at Controlling Bacterial Growth in Bovine Raw Milk Stored at Mild Temperatures

doi: 10.3389/fmicb.2016.00839

Figure Lengend Snippet: Results of the Ryan-Einot-Gabriel-Welsch multiple range test for the three raw milk samples stored at 15°C .

Article Snippet: “Total bacterial counts,” Gram-negative bacteria, lactobacilli and streptococci/lactococci were determined mostly from triplicate (if not duplicate) platings on Plate Count Agar (PCA, LabM, Ltd, Lancashire, UK), MacConkey agar (MC, Becton Dickinson, Le Pont de Claix, France), MRS agar (LabM, Ltd, Lancashire, UK), and M17 agar (Sigma-Aldrich, Steinheim, Germany), respectively, after incubation at 30°C for 3 days under aerobic conditions for PCA and MC plates or at 37°C for 72 h for MRS and M17 plates under anaerobiosis.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Efficiency of N 2 Gas Flushing Compared to the Lactoperoxidase System at Controlling Bacterial Growth in Bovine Raw Milk Stored at Mild Temperatures

doi: 10.3389/fmicb.2016.00839

Figure Lengend Snippet: Results of the Ryan-Einot-Gabriel-Welsch multiple range test for the four raw milk samples stored at 25°C .

Article Snippet: “Total bacterial counts,” Gram-negative bacteria, lactobacilli and streptococci/lactococci were determined mostly from triplicate (if not duplicate) platings on Plate Count Agar (PCA, LabM, Ltd, Lancashire, UK), MacConkey agar (MC, Becton Dickinson, Le Pont de Claix, France), MRS agar (LabM, Ltd, Lancashire, UK), and M17 agar (Sigma-Aldrich, Steinheim, Germany), respectively, after incubation at 30°C for 3 days under aerobic conditions for PCA and MC plates or at 37°C for 72 h for MRS and M17 plates under anaerobiosis.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Efficiency of N 2 Gas Flushing Compared to the Lactoperoxidase System at Controlling Bacterial Growth in Bovine Raw Milk Stored at Mild Temperatures

doi: 10.3389/fmicb.2016.00839

Figure Lengend Snippet: Ratio levels illustrating the incidence of the treatments on bacterial population types enumerated on four considered agar types for raw milk stored at 15°C (A) or at 25°C (B) . PCA, (Plate Count Agar); MC, (MacConkey agar); MRS, (culture of lactic acid bacteria); M17, (lactic streptococci).

Article Snippet: “Total bacterial counts,” Gram-negative bacteria, lactobacilli and streptococci/lactococci were determined mostly from triplicate (if not duplicate) platings on Plate Count Agar (PCA, LabM, Ltd, Lancashire, UK), MacConkey agar (MC, Becton Dickinson, Le Pont de Claix, France), MRS agar (LabM, Ltd, Lancashire, UK), and M17 agar (Sigma-Aldrich, Steinheim, Germany), respectively, after incubation at 30°C for 3 days under aerobic conditions for PCA and MC plates or at 37°C for 72 h for MRS and M17 plates under anaerobiosis.

Techniques: Bacteria

Journal: Frontiers in Microbiology

Article Title: Efficiency of N 2 Gas Flushing Compared to the Lactoperoxidase System at Controlling Bacterial Growth in Bovine Raw Milk Stored at Mild Temperatures

doi: 10.3389/fmicb.2016.00839

Figure Lengend Snippet: Bacterial counts determined for sample M2 stored at 15°C on (A) PCA (Plate Count Agar), (B) MacConkey agar, (C) MRS agar, (D) M17 agar: error bars indicate standard deviations based mainly on three replicates .

Article Snippet: “Total bacterial counts,” Gram-negative bacteria, lactobacilli and streptococci/lactococci were determined mostly from triplicate (if not duplicate) platings on Plate Count Agar (PCA, LabM, Ltd, Lancashire, UK), MacConkey agar (MC, Becton Dickinson, Le Pont de Claix, France), MRS agar (LabM, Ltd, Lancashire, UK), and M17 agar (Sigma-Aldrich, Steinheim, Germany), respectively, after incubation at 30°C for 3 days under aerobic conditions for PCA and MC plates or at 37°C for 72 h for MRS and M17 plates under anaerobiosis.

Techniques:

Journal: Frontiers in Microbiology

Article Title: Efficiency of N 2 Gas Flushing Compared to the Lactoperoxidase System at Controlling Bacterial Growth in Bovine Raw Milk Stored at Mild Temperatures

doi: 10.3389/fmicb.2016.00839

Figure Lengend Snippet: Bacterial counts determined for sample M5 stored at 25°C on (A) PCA (Plate Count Agar), (B) MacConkey agar, (C) MRS agar, (D) M17 agar: error bars indicate standard deviations based mainly on three replicates .

Article Snippet: “Total bacterial counts,” Gram-negative bacteria, lactobacilli and streptococci/lactococci were determined mostly from triplicate (if not duplicate) platings on Plate Count Agar (PCA, LabM, Ltd, Lancashire, UK), MacConkey agar (MC, Becton Dickinson, Le Pont de Claix, France), MRS agar (LabM, Ltd, Lancashire, UK), and M17 agar (Sigma-Aldrich, Steinheim, Germany), respectively, after incubation at 30°C for 3 days under aerobic conditions for PCA and MC plates or at 37°C for 72 h for MRS and M17 plates under anaerobiosis.

Techniques: